W: Group statistics of ion centroids (multi-dataset)
Brief description
Compute ion statistics such as p- and q-values, volcano plots, and many other metrics across groups. This will calculate 'group' statistics to give you an idea of 'significant' features across conditions.
Parameters
Tag (identifier) (type - string)
Tag to use to help identify object.
Centroid tag (type - string)
Tag to use for the object.
Normalization tag (type - string)
Tag to use for normalization.
Help
Rather than applying a normalization to the entire dataset, we apply it as needed to each task at hand.
You can compare the effect normalization has on specific task by repeating it with different normalization.
In some cases, its advised to use 'multi-dataset' normalization, in particular when doing comparisons.
Normalization name (type - string)
Name of the normalization.
Include tags (type - array)
Tags to use for comparisons.
No. of bootstrap samples (type - integer)
Number of bootstrap samples to use for p-value estimation.
Plot style (type - array)
Style of the generated figures.
Export images as (type - string)
Export figures as image or within a single PDF or PowerPoint.
Mask (type - string)
Mask to use for subsampling the data. By default, all pixels will be used for the analysis, otherwise the mask will be used to subsample the data.
Reduce method (type - string)
The method to use for reducing the multi-entry data into a single value. For example. if there are several entries for a single m/z, they need to be simplified to calculate boostrap statistics.
Image reduce method (type - string)
The method used to reduce the amount of image information. By default, each pixel is represented by a single value for each m/z, however, in order to calculate the image statistics, the image must be simplified to a single value. There are a number of methods available for this purpose.
Dependencies (other tasks that this task might depend on)
| Depends on | Required/Optional |
|---|---|
| W: Extract ion centroids (single/multi-dataset) | required |
| W: Extract ion centroids (subset-project) | required |
| P: Normalization (single-dataset) | optional |
| P: Normalization (multi-dataset) | optional |
| P: Normalization (merged-project) | optional |
Attributes
| Attribute | Value | Description |
|---|---|---|
| Multiple allowed | True | Allow multiple instances of this task in a workflow. |
| Task can fail | True | Task is optional and can fail without causing the entire workflow to fail. |
| Step can fail | True | Sub-tasks of this task can fail without causing the entire task (and workflow) to fail. |
| Requires ion mobility | False | Task requires ion mobility data. |
| Task can fail (with ion mobility) | False | This task uses ion mobility data but it is allowed to fail, without causing the entire workflow to fail. |
| Allowed in reference dataset | True | Task is to be performed on a 'reference' dataset. This will allow for multiple analyses to be performed on the same dataset, without cluttering or duplicating certain tasks (unused at the moment). |