W: Filter ion centroids (single-dataset)
Brief description
Filter ion centroids based on m/z, kendrick mass, mass defect, matrix or colocalization metrics.
Task will be performed for each dataset separately, resulting in unique peaklists.
Parameters
Tag (identifier) (type - string)
Tag to use for the object.
Centroid tag (type - string)
Tag to use for the object.
Normalization tag (type - string)
Tag to use for normalization.
Help
Rather than applying a normalization to the entire dataset, we apply it as needed to each task at hand.
You can compare the effect normalization has on specific task by repeating it with different normalization.
In some cases, its advised to use 'multi-dataset' normalization, in particular when doing comparisons.
Normalization name (type - string)
Name of the normalization.
Filter by m/z (type - boolean)
Filter peaklist using mass window, searching for potential isotopes within a specified ~1Da +- ppm window.
PPM tolerance (type - number)
PPM tolerance for filtering by m/z.
Filter by m/z & colocalization (type - boolean)
Filter peaklist using mass window and imate colocalization, searching for potential isotopes within a specified ~1Da +- ppm window and image similarity (measured by colocalization).
PPM tolerance (type - number)
PPM tolerance for filtering by m/z.
Image threshold (type - number)
Threshold for filtering images using the colocalization metric.
Filter by matrix (type - boolean)
Filter peaklist by removing known matrix peaks. Please select appropriate matrix peaklist below
Matrix/Polarity peaklist (type - string)
Matrix or polarity peaklist to use for filtering.
Warning
Filtering by matrix is very much experimental. Matrix peaklists are not finalized and might accidentally remove
valuable signals from your data.
Attributes
| Attribute | Value | Description |
|---|---|---|
| Multiple allowed | True | Allow multiple instances of this task in a workflow. |
| Task can fail | False | Task is optional and can fail without causing the entire workflow to fail. |
| Step can fail | True | Sub-tasks of this task can fail without causing the entire task (and workflow) to fail. |
| Requires ion mobility | False | Task requires ion mobility data. |
| Task can fail (with ion mobility) | False | This task uses ion mobility data but it is allowed to fail, without causing the entire workflow to fail. |
| Allowed in reference dataset | True | Task is to be performed on a 'reference' dataset. This will allow for multiple analyses to be performed on the same dataset, without cluttering or duplicating certain tasks (unused at the moment). |